mail: ysawaあっとlife.shimane-u.ac.jp ("あっと"を"@"に置き換えて下さい)

 ■所属/身分■
島根大学生物資源科学部生命工学科/教授

■研究テーマ■

微生物由来のアミノ酸代謝関連酵素（グルタミン合成酵素アデニリル化調節系、アスパラギン酸脱水素酵素、アミノ基転移酵素、アミノ酸オキシダーゼ）およびバクテリアペルオキシダーゼの構造・機能相関の解明を行うと共に進化分子工学とin sillico解析（分子動力学計算-コンピュータシミュレーション）を用いてこれらの酵素の特性改変（安定性、基質特異性、触媒性変換）のための分子設計を行い、有用物質生産・酵素チップセンサー等への応用を目指している。また、環境中の微生物群集構造の迅速かつ精密な解析手法を確立することを目的として、環境DNAよりPCRで増幅した16s-rDNAについてT-RFLP法 (terminal restriction fragment length polymorphys) の最適分析条件と16s-rDNAデータベース解析システムの構築を行っている。


■著書・総説■

  • Li Y, Ogola, H.J.O, Sawa, Y. L-Aspartate dehydrogenase: features and applications. Appl. Microbiol. Biotechnol. 93: 503-516, 2012.
  • 澤　嘉弘、生化学辞典　第４版、 今堀和友、山川民夫 監修、東京化学同人, ISBN:784807906073, 2007年
  • 澤　嘉弘、光合成微生物の機能と応用, 上原赫 監修、シーエムシー出版, ISBN:4882315963, 2006年


■招待講演■

  • 「L-アスパラギン酸-キノリン酸合成酵素複合体の機能解析」、第65回生物工学会大会 シンポジウム「アミノ酸酸化酵素研究の新展開」、2013年9月、広島市
  • A novel dye-decolorization peroxidase from the cyanobacterium Anabaena sp PCC7120. International Conference on Emerging Trends in Biotechnology, Banaras Hindu University, INDIA, December 4-6, 2009.


■競争的研究資金獲得■

2009-2011文科省特別経費（プロジェクト分）：「未発病状態を判定できる生体指標の開発と島根県特産品の抗酸化性の検証」（代表）

 

■業績リスト(論文)■

  • Azad AK, Hanawa R, Ishikawa T, Sawa Y, Shibata H. Expression profiles of aquaporin homologues and petal movement during petal development in Tulipa gesneriana. Physiol. Plant. doi: 10.1111/j.1399-3054, 2012
  • Yamaguchi M, Sawa Y, Matsuda K, Ozoe F, Ozoe Y. Amino acid residues of both the extracellular and transmembrane domains influence binding of the antiparasitic agent milbemycin to Haemonchus contortus AVR-14B glutamate-gated chloride channels. Biochem. Biophys. Res. Commun. 419: 562-566, 2012.
  • Azad A.K, Yoshikawa N, Ishikawa T, Sawa Y, Shibata H. Substitution of a single amino acid residue in the aromatic/arginine selectivity filter alters the transport profiles of tonoplast aquaporin homologs. Biochim. Biophys. Acta-Biomembranes, 1818: 1-11, 2012.
  • Azad A.K, Jahan M.A, Hasan M.M, Ishikawa T, Sawa Y, Shibata H. Molecular cloning and sequence and 3D models analysis of the Sec61 alpha subunit of protein translocation complex from Penicillium ochrochloron.BMB Reports, 44: 719-724, 2011.
  • Li Y, Ishida M, Ashida H, Ishikawa T, Shibata H, Sawa Y. A Non-NadB Type L-Aspartate Dehydrogenase from Ralstonia eutropha Strain JMP134: Molecular Characterization and Physiological Functions. Biosci. Biotechnol. Biochem. 75: 1524-1532, 2011.
  • Li Y, Kawakami N, Ogola H.J.O, Ashida H, Ishikawa T, Shibata H, Sawa Y. A novel L-aspartate dehydrogenase from the mesophilic bacterium Pseudomonas aeruginosa PAO1: molecular characterization and application for L-aspartate production. Appl. Microbiol. Biotechnol. 90: 1953-1962, 2011.
  • Ogola HJ, Hashimoto N, Miyabe S, Ashida H, Ishikawa T, Shibata H, Sawa Y. Enhancement of hydrogen peroxide stability of a novel Anabaena sp DyP-type peroxidase by site-directed mutagenesis of methionine residues. Appl. Microbiol. Biotechnol. 87: 1727-1736, 2010.
  • Yoshikane Y, Tamura A, Yokochi N, Ellouze K, Yamamura E, Mizunaga H, Fujimoto N, Sakamoto K, Sawa Y, Yagi T. Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with L-glutamate as an amino donor. J. Mol. Cat. B-Enzym. 67: 104-110, 2010.
  • Azad AK, Sato R, Ohtani K, Sawa Y, Ishikawa T, Shibata H. Functional characterization and hyperosmotic regulation of aquaporin in Synechocystis sp PCC 6803. Plant Sci. 180: 375-382, 2010.
  • Ogola HJ, Kamiike T, Hashimoto N, Ashida H, Ishikawa T, Shibata H, Sawa Y. Molecular characterization of a novel peroxidase from the cyanobacterium Anabaena sp. strain PCC 7120. Appl. Environ. Microbiol. 75: 7509-7518, 2009.
  • Azad AK, Sawa Y, Ishikawa T, Shibata H. Heterologous expression of tulip petal plasma membrane aquaporins in Pichia pastoris for water channel analysis. Appl. Environ. Microbiol. 75: 2792-2797, 2009.
  • Azad AK, Ishikawa T, Ishikawa T, Sawa Y, Shibata H. Intracellular energy depletion triggers programmed cell death during petal senescence in tulip. J. Exp. Bot. 59: 2085-2095, 2008.
  • Azad AK, Katsuhara M, Sawa Y, Ishikawa T, Shibata H. Characterization of four plasma membrane aquaporins in tulip petals: a putative homolog is regulated by phosphorylation. Plant Cell Physiol. 49: 1196-1208, 2008.
  • Azad AK, Sawa Y, Ishikawa T, Shibata H. Temperature-dependent stomatal movement in tulip petals controls water transpiration during flower opening and closing. Ann. Appl. Biol. 150: 81-87, 2007.
  • Huang J, Hamasaki T, Ozoe F, Enomoto K, Kataoka H, Sawa Y, Hirota A, Ozoe Y. Identification of critical structural determinants responsible for octopamine binding to the alpha-adrenergic-like Bombyx mori octopamine receptor. Biochemistry 46: 5896-5903, 2007.
  • Azad AK, Y. Sawa, T. Ishikawa, Shibata H. Purification and characterization of protein phosphatase 2A from　petals of Tulipa gesnerina. J. Biochem. Mol. Biol. 39: 671-676, 2006.
  • Ashida H, Sawa Y, Shibata H. Cloning, biochemical and phylogenetic characteriza-tions of gamma-glutamylcysteine synthetase from Anabaena sp PCC 7120. Plant Cell  Physiol. 46: 557-562, 2005.
  • Khan MIH, Kim H, Ashida H, Ishikawa T, Shibata H, Sawa Y. Altering the Substrate Specificity of Glutamate Dehydrogenase from Bacillus subtilis by Site-Directed Mutagenesis. Biosci. Biotechnol. Biochem. 69: 1802-1805, 2005.
  • Khan MIH, Ito K, Kim H, Ashida H, Ishikawa T, Shibata H, Sawa Y. Molecular Properties and Enhancement of Thermostability by Random Mutagenesis of Glutamate Dehy-drogenase from Bacillus subtilis. Biosci. Biotechnol. Biochem. 69: 1861-1870, 2005.
  • Azad AK, Sawa Y, Ishikawa T, Shibata H. Characterization of protein phosphatase 2A acting on phophorylated plasma membrane aqaporin of tulip petals. Biosci. Biotechnol. Biocehem. 68: 1170-1174, 2004.
  • Azad AK,  Sawa Y, Ishikawa T, Shibata H. Phosphorylation of plasma membrane aquaporin regulates temperature-dependent opening of tulip petals. Plant Cell Physiol. 45: 608-617, 2004.
  • Ashida H, Galkin A, Kulakova L, Sawa Y, Nagajima N, Esaki N. Conversion of cofactor specificities of alanine dehydrogenases by site-directed mutagenesis. J. Mol. Cat. B: Enzym. 30: 173-176, 2004.
  • Shibata H, Nagamine T, Wang Y, Ishikawa T, Sawa Y.  Generation of reactive oxygen species from Hinokitiol under near-UV irradiation. Biosci. Biotechnol. Biochem. 67:　1996-1998, 2003.
  • Kim H, Nakaoka M, Yagi M, Ashida H, Hamada K, Shibata H, Sawa Y. Cloning, structural analysis and expression of the gene encoding aspartate aminotransferase from the thermophilic cyanobacterium Phormidium lapideum. J Biosci. Bioeng. 95: 421-424, 2003.
  • Kim H, Ikegami K, Nakaoka M, Yagi M, Shibata H, Sawa Y. Characterization of aspartate aminotransferase from the cyanobacterium Phormidium lapideum. Biosci. Biotechnol. Biochem. 67: 490-498, 2003.
  • Islam SZ, Honda Y, Sawa Y. Characterization of antifungal glycoprotein in red-light-irradiated broadbean leaflets. Mycoscience, 43: 471-473, 2002.
  • Shibata H, Noda N, Ogura Y, Sogabe K, Sawa, Y. Oxidation and reduction of nitrite ion in the TiO2 photo-induced catalytic reaction. Biosci. Biotechnol. Biochem. 64: 1751-1753, 2000.
  • Galkin A. et. al. Cold-adapted alanine dehydrogenases from two antarctic bacterial strains: Gene cloning, protein characterization, and comparison with mesophilic and thermophilic counterparts. Appl. Environ. Microbiol. 65: 4014-4020, 1999.
  • Baker PJ. et al., Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase. Nature Strct. Biol. 5: 561-567, 1998.
  • Sedelnikova S. et al., Crystallization of the alanine dehydrogenase from Phormidium lapideum. Acta Crystall. D. 54: 407-408, 1998.
  • Kono Y. et al., Antioxidant activity of polyphenolics in diets - Rate constants of reactions of chlorogenic acid and caffeic acid with reactive species of oxygen and nitrogen．Biochim. Biophys.  Acta. 1325:  335-342, 1997.
  • Kim KK. et al., Hydroxylation of ent-kaurenoic acid to steviol in Stevia rebaudiana Bertoni - Purification and partial characterization of the enzyme. Archiv. Biochem.  Biophys. 332: 223-230, 1996.
  • Shibata H. et al., A soluble-form of pro-oxidant lumazine isolated from cyanobacterial cells generates superoxide anion under near-UV irradiation.  Bicohim. Biopys. Acta, 1274: 129-134, 1996.
  • Kono Y. et al., Chlorogenic acid as a natural scavenger for hypochlorous acid. Biochem. Biophys. Res. Commun. 217: 972-978, 1995.
  • Kono Y. et al., The suppression of the N-nitrosating reaction by chlorogenic acid. Biochem. J. 312: 947-953, 1995.
  • Sawa Y. et al., Purification and characterization of alanine dehydrogenase from a cyanobacterium, Phormidiium lapideum . J. Biochem. 116: 995-1000, 1994.
  • Brzovic P.S., et al., Evidence that mutations in a loop region of the α-subunit inhibit the transition from an open to a closed conformation in the tryptophan synthase bienzyme complex. J. Biol. Chem., 267: 13028-13038, 1992.
  • Kayastha A.M., et al., Site-directed mutagenesis of the β-subunit of tryptophan synthase from Salmonella typhimurium – Role of Active-site glutamic acid-350 . J. Biol. Chem., 266: 7618-7625, 1991.
  • Sawa Y., et al., Glutamine synthetase from a cyanobacterium Phormidium lapideum. J. Biochem. 104: 917-923, 1988.
  • Ochiai H., et al., Living electrode as a long-lived photoconverter for biophotolysis of water. PNAS. USA. 77: 2442-2444, 1980.